Volume: 1.1.2
Investigation of the ameliorative effects of gallic acid against neurotoxicity caused by glutamate in C6 cells
Adem AHLATCI
Vocational School of Health Services, Van Yuzuncu Yil University, Van, Türkiye
Abstract
Gallic acid (GA) is present as a phenolic component of various foods and plants. GA is a molecule with broad biological properties such as antioxidant, anti-microbial, and anti-inflammatory activities. As the main excitatory neurotransmitter in the mammalian central nervous system excessive extracellular glutamate can activate the glutamate receptors and neuronal/intracellular calcium (Ca2+) overload, producing neurotoxicity, a common pathway for neuronal injury or death and is associated with neurodegenerative diseases. The present study aimed to investigate the effect of gallic acid on glutamate-induced cytotoxicity in C6 glioma cells. For the study, groups were formed from C6 cells as control, GA (100 µM, 24 h), Glutamate (10 mM, 24 h), and GA+Glutamate. In the study, Total oxidant (TOS), total antioxidant (TAS), MDA, and caspase-3 levels in the cells were determined by ELISA kit. The results showed that glutamate administration increased TOS, MDA, and caspase-3 levels by causing cytotoxicity in C6 cells (p<0.05). However, in C6 cells treated with GA before glutamate incubation, TOS, MDA, and caspase-3 levels were decreased, and TAS levels increased compared to the glutamate group (p<0.05). As a result, it was determined that GA treatment showed a protective effect in the glutamate-induced cytotoxicity model in C6 cells.
Keywords: Gallic acid, Glutamate, Oxidative stress, caspase-3, C6 glioma cells
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