{"id":519,"date":"2024-08-31T18:35:24","date_gmt":"2024-08-31T15:35:24","guid":{"rendered":"https:\/\/ncmrjournal.com.tr\/?p=519"},"modified":"2024-08-31T22:24:52","modified_gmt":"2024-08-31T19:24:52","slug":"vol-3","status":"publish","type":"post","link":"https:\/\/ncmrjournal.com.tr\/index.php\/2024\/08\/31\/vol-3\/","title":{"rendered":"Volume: 1.2.2"},"content":{"rendered":"<h5><strong>TRPM2 channel contribution to CdCI<sub>2<\/sub>-related neurotoxicity in SH-SY5Y cells: Protective role of selenium<\/strong><\/h5>\n<p><strong>Ramazan \u00c7INAR<sup>1<\/sup>, Adem AHLATCI<sup>2*<\/sup><\/strong><\/p>\n<p><sup>1<\/sup>Department of Biophysics, Faculty of Medicine, Bilecik Seyh Edebali University, Bilecik, T\u00fcrkiye<\/p>\n<p><sup>2<\/sup>Vocational School of Health Services, Van Yuzuncu Yil University, Van, T\u00fcrkiye<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Abstract<\/strong><\/p>\n<p>Humans are most exposed to the heavy metal cadmium (Cd), known as neurotoxic. However, it is unclear how selenium (Se) protects neurons from damage caused by increased Cd-induced neurotoxicity in SH-SY5Y cells and how the TRPM2 channel functions in this process. In this study, we examined the impact of Se on CdCI<sub>2<\/sub>-induced oxidative neurotoxicity and cell death in SH-SY5Y cells by modifying the TRPM2 channel. The Se and TRPM2 channel antagonist 2-APB was added to prevent CdCI<sub>2<\/sub>-induced neurotoxicity in SH-SY5Y cells. Cell viability rate was determined between groups by CCK-8 assay. GSH, MDA, and ROS levels were determined in the cells with ELISA kits. Our results showed that the TRPM2 channel plays a vital role in forming CdCI<sub>2<\/sub>-induced damage to cells by using the TRPM2 antagonist in the study. We also observed that Se reduced CdCI<sub>2<\/sub>-induced neurotoxicity by reducing TRPM2 channel activation by suppressing oxidative stress of cells. We conclude that Se therapy and TRPM2 channel blocking can reduce CdCI<sub>2<\/sub>-induced neurotoxicity based on our investigation, which examined the protective impact of Se and the involvement of the TRPM2 channel in CdCI<sub>2<\/sub>-induced SH-SY5Y cells for the first time.<\/p>\n<p><strong>Keywords:<\/strong> Cadmium, Selenium, SH-SY5Y cells, TRPM2 channel<\/p>\n<p><strong style=\"font-size: 1.5rem;\"><em>Page; 40-46\u00a0 \u00a0<\/em><\/strong><span style=\"font-size: 1.5rem;\">\u00a0 <\/span><a style=\"font-size: 1.5rem;\" href=\"https:\/\/ncmrjournal.com.tr\/wp-content\/uploads\/2024\/08\/vol-1.2.2.pdf\" target=\"_blank\" rel=\"noopener\"><strong>PDF<\/strong><\/a><\/p>\n<p><strong><em>DOI: <a href=\"https:\/\/doi.org\/10.5281\/zenodo.13623107\">https:\/\/doi.org\/10.5281\/zenodo.13623107<\/a><\/em><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>TRPM2 channel contribution to CdCI2-related neurotoxicity in SH-SY5Y cells: Protective role of selenium Ramazan \u00c7INAR1, Adem AHLATCI2* 1Department of Biophysics, Faculty of Medicine, Bilecik Seyh Edebali University, Bilecik, T\u00fcrkiye 2Vocational School of Health Services, Van Yuzuncu Yil University, Van, T\u00fcrkiye &nbsp; Abstract Humans are most <a class=\"more-link\" href=\"https:\/\/ncmrjournal.com.tr\/index.php\/2024\/08\/31\/vol-3\/\">Read More &#8230;<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-519","post","type-post","status-publish","format-standard","hentry","category-genel"],"_links":{"self":[{"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/519","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/comments?post=519"}],"version-history":[{"count":6,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/519\/revisions"}],"predecessor-version":[{"id":533,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/519\/revisions\/533"}],"wp:attachment":[{"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/media?parent=519"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/categories?post=519"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/tags?post=519"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}