{"id":349,"date":"2024-05-30T16:02:40","date_gmt":"2024-05-30T13:02:40","guid":{"rendered":"https:\/\/ncmrjournal.com.tr\/?p=349"},"modified":"2024-06-03T22:37:21","modified_gmt":"2024-06-03T19:37:21","slug":"volume-1-1-1-ayku-m","status":"publish","type":"post","link":"https:\/\/ncmrjournal.com.tr\/index.php\/2024\/05\/30\/volume-1-1-1-ayku-m\/","title":{"rendered":"Volume: 1.1.1"},"content":{"rendered":"<h5><strong>Bioinformatics analysis of the presence of Ca<sup>2+<\/sup> channels of <em>Acanthamoeba castellanii<\/em><\/strong><\/h5>\n<p><strong>Mehmet AYKUR<\/strong><\/p>\n<p>Department of Parasitology, Tokat Gaziosmanpa\u015fa University, Faculty of Medicine, Tokat, T\u00fcrkiye<\/p>\n<p><strong>Abstract<\/strong><\/p>\n<p>The free-living amoeba (FLA) belonging to the genus <em>Acanthamoeba<\/em> is the most widespread protozoan in the environment, found in natural and man-made environments. <em>Acanthamoeba<\/em> is an opportunistic protist capable of causing granulomatous amebic encephalitis (GAE), a fatal disease of the central nervous system (CNS), and <em>Acanthamoeba<\/em> keratitis (AK), a painful, progressive, and sight-threatening infection. A single drug that can simultaneously eliminate both trophozoite and cystic forms of the parasite is currently unavailable. Bioinformatics research has aimed to reveal the presence of new therapeutic targets and pathways in the treatment of <em>Acanthamoeba<\/em>.<\/p>\n<p>Calcium (Ca<sup>2+<\/sup>) channels regulate many vital functions within cells. Ca<sup>2+ <\/sup>influx mediates the regulation of physiological signaling pathways in parasites. Calcium channels in <em>Acanthamoeba<\/em> allow the intracellular Ca<sup>2+<\/sup> stores to be refilled following intracellular Ca<sup>2+<\/sup> release. It is also well known that the role of calcium in the activation of some anti-parasitic drugs is very important. The search for protein sequence homology between two-pore calcium channel protein 1 (TPC-1), TPC-2, and calmodulin was done by searching the <em>Acanthamoeba<\/em> Neff strain protein databases at NCBI and amoebadb.org by using BLAST<em>p<\/em> search. The BLAST<em>p<\/em> alignment option was selected to show similarities between the proteins of both species.<\/p>\n<p>In conclusion, it may be an option for a narrow treatment approach for GAE and AK caused by <em>Acanthamoeba<\/em> species; therefore, the discovery of calcium channels (TPC-1, TPC-2, and Calmodulin) in <em>Acanthamoeba<\/em> could prove to be a potential therapeutic target in the future.<\/p>\n<p><strong>Keywords: <\/strong>Bioinformatics tools, Acanthamoeba castellanii, Ca<sup>2+<\/sup> channels, new therapeutic targets<\/p>\n<p><strong><em>Page; 1-6\u00a0 \u00a0<\/em><\/strong>\u00a0 <a href=\"https:\/\/ncmrjournal.com.tr\/wp-content\/uploads\/2024\/06\/ncmr-vol-1.1.1.pdf\" target=\"_blank\" rel=\"noopener\"><strong>PDF<\/strong><\/a><\/p>\n<p><strong><em>DOI: <a href=\"https:\/\/doi.org\/10.5281\/zenodo.11455721\">https:\/\/doi.org\/10.5281\/zenodo.11455721<\/a>\u00a0<\/em><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Bioinformatics analysis of the presence of Ca2+ channels of Acanthamoeba castellanii Mehmet AYKUR Department of Parasitology, Tokat Gaziosmanpa\u015fa University, Faculty of Medicine, Tokat, T\u00fcrkiye Abstract The free-living amoeba (FLA) belonging to the genus Acanthamoeba is the most widespread protozoan in the environment, found in natural <a class=\"more-link\" href=\"https:\/\/ncmrjournal.com.tr\/index.php\/2024\/05\/30\/volume-1-1-1-ayku-m\/\">Read More &#8230;<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-349","post","type-post","status-publish","format-standard","hentry","category-vol-1-1"],"_links":{"self":[{"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/349","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/comments?post=349"}],"version-history":[{"count":8,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/349\/revisions"}],"predecessor-version":[{"id":385,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/349\/revisions\/385"}],"wp:attachment":[{"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/media?parent=349"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/categories?post=349"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ncmrjournal.com.tr\/index.php\/wp-json\/wp\/v2\/tags?post=349"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}